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KMID : 0616120160470010043
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Korean Journal of Pharmacognosy
2016 Volume.47 No. 1 p.43 ~ p.48
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Anticariogenic Activity from Purified Bee Venom (Apis mellifera L.) against Four Cariogenic Bacteria
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Han Sang-Mi
Hong In-Pyo
Woo Soon-Ok
Park Kwan-Kyu
Chang Young-Chae
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Abstract
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The aim of the study was performed to examine the anticariogenic potential of purified bee venom (Apis mellifera L., PBV) collected using bee venom collector from cariogenic bacteria, Streptococcus mutans, Streptococcus sanguis, Porphyromonas gingivalis, and Fusobacterium nucleatum. The anticariogenic effect of purified bee venom was evaluated by agar well diffusion method, minimum inhibitory concentraion (MIC), minimum bactericidal concentration (MBC), and postantibiotic effect (PAE). The human lower gingiva epithelial cell cytotoxicity of purified bee venom was also evaluated. Purified bee venom exhibited significant inhibition of bacterial growth of S. mutans, S. sanguis, P. gingivalis, and F. nucleatum with MIC value of 0.68, 0.85, 3.49, and 2.79 mg/ml , respectively. The MBC value of purified bee venom against S. mutans, S. sanguis, P. gingivalis, and F. nucleatum was 1.34, 1.67, 8.5, and 6.8 mg/ml. Furthermore, the results of PAE values against S. mutans, S. sanguis, P. gingivalis, and F. nucleatum showed the bacterial effect with 3.3, 3.45, 2.0, and 2.0. The concentration below 1 mg/ml of purified bee venom had no cytotoxicity in the human lower gingiva epithelial cell. These results suggested that purified bee venom have great potenial as anticariogenic agents.
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KEYWORD
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Apis mellifera , Purified bee venom , Cariogenic bacteria , Antibacterial activity , Gingiva epithelial cell
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